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Prometheus NT.48 Nano Temper Technologies Protein Foling Analyser

Condition Used
Stock Number 45360 / 1500
This listing is for the Prometheus NT.48 Nano Temper Technologies Protein Foling Analyser

The unit is in full working condition and is ready for immediate release


The Prometheus NT.48 is a cutting-edge, high-resolution instrument used for protein stability characterization. It utilizes a variety of label-free technologies to monitor both thermal and chemical stability, making it an invaluable tool in drug development, particularly for protein-based therapeutics like antibodies.


Here's a breakdown of its key features and functionalities:


Core Features:
Native DSF (Differential Scanning Fluorimetry): No dyes required for detection. It measures protein stability by detecting intrinsic fluorescence (tryptophan and tyrosine) during thermal or chemical denaturation.
Dual UV System: Uses two UV detectors to monitor fluorescence at 330 nm and 350 nm, providing high scanning speed and dense data points for ultra-high resolution measurements.
High-Resolution Detection: Capable of measuring subtle changes in unfolding transitions, even at very high concentrations or complex samples. This is crucial for detecting aggregation and monitoring thermal stability.
Sample Throughput: Can handle 48 samples simultaneously, making it suitable for high-throughput experiments such as buffer screening or multi-step profiling.
Broad Concentration Range: Works with samples ranging from 5 µg/mL to 250 mg/mL, making it suitable for a wide variety of applications, including early-phase antibody development and final formulation screening.


Temperature Range: From 15 °C to 95 °C, allowing for detailed temperature-dependent stability analysis
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Easy Handling: Requires only 10 µL of sample per condition, and features an intuitive software interface for easy operation and data analysis.
Maintenance-Free: Designed with capillary-based measurements for easy sample handling and durability, even under challenging sample conditions such as high viscosity formulations.
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Advanced Features:
Backreflection Optics (Optional): Enhances the ability to detect aggregation in protein samples by measuring light scattering, particularly useful for studying protein aggregation in formulations.
High Sensitivity and Reproducibility: The capillary format and automatic internal referencing contribute to the instrument’s exceptional sensitivity and reproducibility, particularly for aggregation detection.
Wide Application: Can be used for both thermal and chemical denaturation and can analyze detergent-solubilized membrane proteins, highly concentrated formulations, and other complex biological samples.
Software and Compliance: Equipped with rmControl and bility Analysis software, supporting 21 CFR Part 11 compliance for use in regulated environments.
Applications:
Thermal Unfolding: Studies how proteins unfold as temperature increases, which can be crucial in drug design.
Chemical Unfolding: Measures stability in the presence of chemical denaturants, helping to identify compounds that destabilize proteins.
Aggregation Detection: Identifies the onset of aggregation, providing insights into protein formulation stability and quality control.
Protein Engineering and Formulation Screening: Ideal for optimizing protein formulations and screening for the best candidates based on stability data.